|
|
|
|
|
| Melo,Ligia Maria Rodrigues de; Almeida,Dulce; Hofer,Ernesto; Reis,Cristhiane Moura Falavina dos; Theophilo,Grace Nazareth Diogo; Santos,André Felipe das Mercês; Vieira,Regine Helena Silva dos Fernandes. |
| Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio parahaemolyticus; Litopenaeus vannamei; Multiple antibiotic resistance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400032 |
| |
|
|
| Lima e Silva,Agostinho A. de; Carvalho,Márcia A. Ribeiro de; Souza,Sérgio A. L de; Dias,Patrícia M. Teixeira; Silva Filho,Renato G. da; Saramago,Carmen S. de Meirelles; Bento,Cleonice A. de Melo; Hofer,Ernesto. |
| Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metal tolerance; Sewage; Pigment; Enzymatic inhibition; Antimicrobial resistance. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047 |
| |
|
|
| Rusak,Leonardo Alves; Junqueira,Ricardo Magrani; Hofer,Ernesto; Vallim,Deyse Christina; Asensi,Marise Dutra. |
| Abstract Yersinia enterocolitica is a widespread Gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. Potentially pathogenic Y. enterocolitica has been isolated in Brazil, from human, environmental, food, and animal sources. Herein we report a genome sequence of Y. enterocolitica subsp. palearctica strain YE 19, serotype O:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in Rio de Janeiro in 2005. The results corroborate other findings that this strain harbors a set of virulence determinants that could play a role in host pathoadaptation and may also justify the successful dissemination of bioserotype 4/O:3 in Brazil. The presence of strains harboring all of these... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Yersinia enterocolitica; Virulence; Brazil; Next-generation sequencing. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500550 |
| |
|
| |
|
|
| Almeida,Rosana Macedo de; Barbosa,André Victor; Lisbôa,Rodrigo de Castro; Santos,André Felipe das Mercês; Hofer,Ernesto; Vallim,Deyse Christina; Hofer,Cristina Barroso. |
| ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Listeriosis; Virulence; Food; PFGE; Brazil. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282 |
| |
|
|
|
